p stat2 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
p stat2
P Stat2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+phospho+stat2/Phospho-Stat2+(Tyr690)+Rabbit+mAb/pmc13039202-279-60-80
Average 96 stars, based on 186 article reviews
P Stat2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+phospho+stat2/Phospho-Stat2+(Tyr690)+Rabbit+mAb/pmc13039202-279-60-80
Average 96 stars, based on 186 article reviews
p stat2 - by Bioz Stars,
2026-09
96/100 stars
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Staining:Article Title: Trichomonas vaginalis extracellular vesicles suppress IFNε-mediated responses driven by its intracellular bacterial symbiont Mycoplasma hominis . Article Snippet: Luciferase reporter cells were treated with 10 μg/mL of B7RC2 TvEVs for 6 h and then stimulated with IFNε (500 ng/mL) for another 6 h. The cells were then washed and assayed for expression of Gaussian Luciferase in cell lysates using a BioLux Gaussia Luciferase Assay Kit (New England BioLabs) as per the manufacturer’s instructions. pSTAT1 and pSTAT2 Nuclear Translocation by Immunofluorescence. .. BPH- 1 or Ect1 cells were treated with either TvEVs (50 μg/mL) or mock treated for 6 h and then stimulated with IFNε (500 ng/mL) for 1 h. The cells were fixed with 100% methanol for 15 min and blocked with 5% w/v BSA for 1 h. Fixed cells were stained with 1:200 rabbit anti- phospho- STAT1 (Cell Signaling Technologies; Cat # 9167S) or 1:200 Article Title: Trichomonas vaginalis extracellular vesicles suppress IFNε-mediated responses driven by its intracellular bacterial symbiont Mycoplasma hominis Article Snippet: Luciferase reporter cells were treated with 10 μg/mL of B7RC2 TvEVs for 6 h and then stimulated with IFNε (500 ng/mL) for another 6 h. The cells were then washed and assayed for expression of Gaussian Luciferase in cell lysates using a BioLux Gaussia Luciferase Assay Kit (New England BioLabs) as per the manufacturer’s instructions. .. BPH-1 or Ect1 cells were treated with either TvEVs (50 μg/mL) or mock treated for 6 h and then stimulated with IFNε (500 ng/mL) for 1 h. The cells were fixed with 100% methanol for 15 min and blocked with 5% w/v BSA for 1 h. Fixed cells were stained with 1:200 rabbit anti-phospho-STAT1 (Cell Signaling Technologies; Cat # 9167S) or 1:200 High Throughput Screening Assay:Article Title: Trichomonas vaginalis extracellular vesicles suppress IFNε-mediated responses driven by its intracellular bacterial symbiont Mycoplasma hominis . Article Snippet: Luciferase reporter cells were treated with 10 μg/mL of B7RC2 TvEVs for 6 h and then stimulated with IFNε (500 ng/mL) for another 6 h. The cells were then washed and assayed for expression of Gaussian Luciferase in cell lysates using a BioLux Gaussia Luciferase Assay Kit (New England BioLabs) as per the manufacturer’s instructions. pSTAT1 and pSTAT2 Nuclear Translocation by Immunofluorescence. .. BPH- 1 or Ect1 cells were treated with either TvEVs (50 μg/mL) or mock treated for 6 h and then stimulated with IFNε (500 ng/mL) for 1 h. The cells were fixed with 100% methanol for 15 min and blocked with 5% w/v BSA for 1 h. Fixed cells were stained with 1:200 rabbit anti- phospho- STAT1 (Cell Signaling Technologies; Cat # 9167S) or 1:200 Imaging:Article Title: Trichomonas vaginalis extracellular vesicles suppress IFNε-mediated responses driven by its intracellular bacterial symbiont Mycoplasma hominis . Article Snippet: Luciferase reporter cells were treated with 10 μg/mL of B7RC2 TvEVs for 6 h and then stimulated with IFNε (500 ng/mL) for another 6 h. The cells were then washed and assayed for expression of Gaussian Luciferase in cell lysates using a BioLux Gaussia Luciferase Assay Kit (New England BioLabs) as per the manufacturer’s instructions. pSTAT1 and pSTAT2 Nuclear Translocation by Immunofluorescence. .. BPH- 1 or Ect1 cells were treated with either TvEVs (50 μg/mL) or mock treated for 6 h and then stimulated with IFNε (500 ng/mL) for 1 h. The cells were fixed with 100% methanol for 15 min and blocked with 5% w/v BSA for 1 h. Fixed cells were stained with 1:200 rabbit anti- phospho- STAT1 (Cell Signaling Technologies; Cat # 9167S) or 1:200 Article Title: Trichomonas vaginalis extracellular vesicles suppress IFNε-mediated responses driven by its intracellular bacterial symbiont Mycoplasma hominis Article Snippet: Luciferase reporter cells were treated with 10 μg/mL of B7RC2 TvEVs for 6 h and then stimulated with IFNε (500 ng/mL) for another 6 h. The cells were then washed and assayed for expression of Gaussian Luciferase in cell lysates using a BioLux Gaussia Luciferase Assay Kit (New England BioLabs) as per the manufacturer’s instructions. .. BPH-1 or Ect1 cells were treated with either TvEVs (50 μg/mL) or mock treated for 6 h and then stimulated with IFNε (500 ng/mL) for 1 h. The cells were fixed with 100% methanol for 15 min and blocked with 5% w/v BSA for 1 h. Fixed cells were stained with 1:200 rabbit anti-phospho-STAT1 (Cell Signaling Technologies; Cat # 9167S) or 1:200 Software:Article Title: Trichomonas vaginalis extracellular vesicles suppress IFNε-mediated responses driven by its intracellular bacterial symbiont Mycoplasma hominis . Article Snippet: Luciferase reporter cells were treated with 10 μg/mL of B7RC2 TvEVs for 6 h and then stimulated with IFNε (500 ng/mL) for another 6 h. The cells were then washed and assayed for expression of Gaussian Luciferase in cell lysates using a BioLux Gaussia Luciferase Assay Kit (New England BioLabs) as per the manufacturer’s instructions. pSTAT1 and pSTAT2 Nuclear Translocation by Immunofluorescence. .. BPH- 1 or Ect1 cells were treated with either TvEVs (50 μg/mL) or mock treated for 6 h and then stimulated with IFNε (500 ng/mL) for 1 h. The cells were fixed with 100% methanol for 15 min and blocked with 5% w/v BSA for 1 h. Fixed cells were stained with 1:200 rabbit anti- phospho- STAT1 (Cell Signaling Technologies; Cat # 9167S) or 1:200 Article Title: Trichomonas vaginalis extracellular vesicles suppress IFNε-mediated responses driven by its intracellular bacterial symbiont Mycoplasma hominis Article Snippet: Luciferase reporter cells were treated with 10 μg/mL of B7RC2 TvEVs for 6 h and then stimulated with IFNε (500 ng/mL) for another 6 h. The cells were then washed and assayed for expression of Gaussian Luciferase in cell lysates using a BioLux Gaussia Luciferase Assay Kit (New England BioLabs) as per the manufacturer’s instructions. .. BPH-1 or Ect1 cells were treated with either TvEVs (50 μg/mL) or mock treated for 6 h and then stimulated with IFNε (500 ng/mL) for 1 h. The cells were fixed with 100% methanol for 15 min and blocked with 5% w/v BSA for 1 h. Fixed cells were stained with 1:200 rabbit anti-phospho-STAT1 (Cell Signaling Technologies; Cat # 9167S) or 1:200 Incubation:Article Title: Intertumoral heterogeneity impacts oncolytic vesicular stomatitis virus efficacy in mouse pancreatic cancer cells. Article Snippet: .. Membranes were then incubated in TBS-T with 5% BSA or milk with 0.02% sodium azide and a 1:5,000 dilution of rabbit polyclonal anti-VSV antibodies (raised against VSV virions), a 1:1,000 dilution of rabbit anti-phospho-STAT1 [catalog number 9177S, P-STAT1 (S727) Cell Signaling], a 1:1,000 dilution of rabbit anti-STAT1 (catalog number 14994T, D1K9Y, Cell Signaling), a 1:1,000 dilution of Article Title: Non-Canonical Role of IKKα in the Regulation of STAT1 Phosphorylation in Antiviral Signaling Article Snippet: The proteins were then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA), which were subsequently blocked for 1 h at room temperature in TBST buffer [20 mM Tris (pH 7.4), 150 mM NaCl, and 0.1% Tween 20] containing 5% nonfat dry milk (blocking buffer). .. Next, the membranes were incubated overnight at 4°C with one of the following primary antibodies: rabbit anti-IKKα, rabbit anti-IKKβ, rabbit anti-IKKγ, rabbit anti-JAK1, rabbit anti-Tyk2, rabbit anti-phospho-JAK1, rabbit anti-phospho-Tyk2, rabbit anti-p50, rabbit anti-p52, rabbit anti-Rel B, rabbit anti-IκBα, |
